18295 1 ap Search Results


timeless 961a  (Proteintech)
95
Proteintech timeless 961a
(A) Schematic of PARP1-mediated TIM PARylation and PAR-dependent proteolysis. (B-D) WB of U2OS parental or PARP1 KO clones transduced with <t>pBABE-HRAS</t> G12V (vs. EV) for 24 h. Where indicated, 10 μM olaparib (ola) or talazoparib (tal) was co-treated for 20 h before harvest. (E) WB of IMR90 ER:HRAS G12V induced by 100 nM 4-OHT in the presence or absence of 10 μM olaparib. (F, G) WB of BJ-5ta ER:HRAS G12V induced by 4-OHT. For (G), cells were co-treated with indicated inhibitors for 16 h. (H) Anti-Flag immunoprecipitation (IP) of Flag-TIM (pcDNA4) in denaturing conditions followed by anti-pADPr WB in U2OS ER:HRAS G12V induced by 4-OHT and treated with indicated inhibitors for 16 h. (I) As (H), but in cells expressing Flag-TIM wild-type (WT) or PBM1/2. (J) A model depicting the signaling cascade that connects oncogenic RAS to PARP1, prompting PAR-dependent proteasomal degradation of TIM.
Timeless 961a, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/18295+1+ap/HRAS-Specific+Antibody/pmc12813712-32-11-12
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timeless 961a - by Bioz Stars, 2026-10
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(A) Schematic of PARP1-mediated TIM PARylation and PAR-dependent proteolysis. (B-D) WB of U2OS parental or PARP1 KO clones transduced with pBABE-HRAS G12V (vs. EV) for 24 h. Where indicated, 10 μM olaparib (ola) or talazoparib (tal) was co-treated for 20 h before harvest. (E) WB of IMR90 ER:HRAS G12V induced by 100 nM 4-OHT in the presence or absence of 10 μM olaparib. (F, G) WB of BJ-5ta ER:HRAS G12V induced by 4-OHT. For (G), cells were co-treated with indicated inhibitors for 16 h. (H) Anti-Flag immunoprecipitation (IP) of Flag-TIM (pcDNA4) in denaturing conditions followed by anti-pADPr WB in U2OS ER:HRAS G12V induced by 4-OHT and treated with indicated inhibitors for 16 h. (I) As (H), but in cells expressing Flag-TIM wild-type (WT) or PBM1/2. (J) A model depicting the signaling cascade that connects oncogenic RAS to PARP1, prompting PAR-dependent proteasomal degradation of TIM.

Journal: Biochemical and biophysical research communications

Article Title: Loss of DNA replication fork protection by TIMELESS degradation supports oncogene-induced senescence

doi: 10.1016/j.bbrc.2025.152203

Figure Lengend Snippet: (A) Schematic of PARP1-mediated TIM PARylation and PAR-dependent proteolysis. (B-D) WB of U2OS parental or PARP1 KO clones transduced with pBABE-HRAS G12V (vs. EV) for 24 h. Where indicated, 10 μM olaparib (ola) or talazoparib (tal) was co-treated for 20 h before harvest. (E) WB of IMR90 ER:HRAS G12V induced by 100 nM 4-OHT in the presence or absence of 10 μM olaparib. (F, G) WB of BJ-5ta ER:HRAS G12V induced by 4-OHT. For (G), cells were co-treated with indicated inhibitors for 16 h. (H) Anti-Flag immunoprecipitation (IP) of Flag-TIM (pcDNA4) in denaturing conditions followed by anti-pADPr WB in U2OS ER:HRAS G12V induced by 4-OHT and treated with indicated inhibitors for 16 h. (I) As (H), but in cells expressing Flag-TIM wild-type (WT) or PBM1/2. (J) A model depicting the signaling cascade that connects oncogenic RAS to PARP1, prompting PAR-dependent proteasomal degradation of TIM.

Article Snippet: Following antibodies were used: TIMELESS (Bethyl, A300–961A), RB (Santa Cruz, sc-102), HRAS (Proteintech, CL488–18295), Poly(ADP-ribose) (R&D, 4336-BPC-100), PCNA (Santa Cruz, sc-56), Cyclin A (Santa Cruz, sc-271682), and anti-Flag M2 affinity gel (Sigma, A2220).

Techniques: Activation Assay, Clone Assay, Transduction, Immunoprecipitation, Expressing